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宿主来源
Human抗原名称
F4/80分子别名
Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; Emr1; Gpf480; Adgre1细胞定位
Cell membraneAccession
Q61549克隆号
S-3458抗体类型
Recombinant mAb抗体同种型
IgG1,k反应种属 ?
Ms阳性样本
BALB/c mouse peritoneal exudates cells纯化方式
Protein A浓度
2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1:2000 Ms
F4/80, also known as ADGRE1 or EMR1, is a cell surface glycoprotein and a member of the EGF-TM7 protein family. It is widely used as a marker for mature mouse macrophages and is highly expressed in various macrophage populations, such as Kupffer cells in the liver, Langerhans cells in the skin, and microglial cells in the brain. However, its expression in human cells is different, being mainly found in eosinophils and some macrophages. F4/80 is an orphan receptor involved in cell adhesion and immune cell interactions, and it may also play a role in the development of regulatory T cells. The protein has a large extracellular domain containing multiple EGF-like calcium-binding domains, linked to a seven-transmembrane domain characteristic of G protein-coupled receptors.
流式分析
Flow cytometric analysis of BALB/c mouse peritoneal exudates cells labelling Mouse F4/80 antibody at 1/2000 dilution (0.1 μg) / (Right panel) compared with a Human IgG1, λ Isotype Control / (Left panel). Goat Anti-Human IgG Alexa Fluor® 488 was used as the secondary antibody.Then cells were stained with CD11b - Brilliant Violet 421™ Antibody separately.
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