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宿主来源
Rabbit抗原名称
CD9分子别名
CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein (MRP-1); Tetraspanin-29 (Tspan-29); p24; MIC3; TSPAN29免疫原
Recombinant Protein细胞定位
Cell membraneAccession
P21926克隆号
S-143-53抗体类型
Recombinant mAb抗体同种型
IgG同型对照
S0B8556反应种属 ?
Hu阳性样本
Fresh human peripheral blood cells纯化方式
Protein A浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD9, a 24-kDa cell-surface glycoprotein belonging to the tetraspanin family, is characterized by four transmembrane domains and two extracellular loops; it is widely expressed (e.g., on platelets, B/T cells, macrophages, and oocytes) and organizes tetraspanin-enriched microdomains that integrate membrane and cytoplasmic proteins to regulate adhesion, migration, proliferation, survival, and immune synapse formation. Notably, CD9 is essential for mammalian gamete fusion, as CD9-null eggs cannot fuse with wild-type sperm, and anti-CD9 antibodies block sperm–egg binding and fusion in vitro. Additionally, CD9 is implicated in platelet activation, signal transduction, and is frequently detected in lymphoblastic leukemia/lymphoma and some acute myeloid leukemia cells.
流式分析
Flow cytometric analysis of CD9 expression on fresh human peripheral blood cells. Fresh human peripheral blood cells were stained with APC Mouse Anti-Human CD41 Antibody and either FITC Rabbit IgG Isotype Control (left panel) or SDT FITC Rabbit Anti-Human CD9 Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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