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产品中心 流式试剂&相关 流式抗体

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FITC Rat IgG2b Isotype Control (S-R486)

价格 ￥350.00 （ 1-2周）
货号 S0B8071
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产品介绍

产品介绍 评论(0)

产品规格

  • 宿主来源

    Rat

  • 克隆号

    S-R486

  • 抗体类型

    Rat mAb

  • 抗体同种型

    IgG2b

  • 纯化方式

    Protein G

  • 浓度

    0.2 mg/ml

  • 标记

    FITC

  • 性状

    Liquid

  • 缓冲体系

    PBS, 1% BSA, 0.3% Proclin 300

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8 °C as supplied

  • 应用

    ICFCM

    FCM

背景介绍

  • Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.

  • 流式分析

    • BALB/c mouse splenocytes were stained with SDT FITC Rat IgG2b Isotype Control Antibody (Right panel) at 1/20 dilution (1 μg). Cells without incubation with primary antibody and secondary antibody (Left panel) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software

    • Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) labelling SDT FITC Rat IgG2b Isotype Control Antibody at 1/20 dilution (1 μg) (Red line histogram) compared with unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue line histogram). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.

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