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宿主来源
Mouse抗原名称
CD274分子别名
Programmed cell death 1 ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; hPD-L1; B7 homolog 1 (B7-H1); B7H1; PDCD1L1; PDCD1LG1; PDL1细胞定位
Nucleus, Cell membraneAccession
Q9NZQ7克隆号
MIH1抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
PHA stimulated human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD274, also known as PD-L1 (Programmed Death-Ligand 1), is a ~33 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily that functions as the key ligand for the inhibitory receptor PD-1 on T cells; upon IFN-γ–driven up-regulation on dendritic cells, macrophages, normal epithelia and many tumors, its engagement of PD-1 delivers a “stop” signal that blocks T-cell receptor signaling, suppressing proliferation, cytokine production and cytotoxic activity, thereby maintaining peripheral tolerance and preventing autoimmunity, but is exploited by cancers to evade immune destruction, making the PD-1/PD-L1 axis a central target for modern checkpoint-inhibitor immunotherapies.
流式分析
Flow cytometric analysis of human PBMC were stained with stimulated 2 days with 10 μg/ml PHA (Right panel) or unstimulated (Left panel) was stained with Brilliant Violet 421™ Mouse Anti-Human CD3 and SDT FITC Mouse Anti-Human CD274 Antibody Antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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