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宿主来源
Mouse抗原名称
MICA分子别名
MHC class I polypeptide-related sequence A; MIC-A; PERB11.1细胞定位
Cytoplasm, Cell membrane, Endoplasmic reticulumAccession
Q29983克隆号
S-2794抗体类型
Mouse mAb抗体同种型
IgG1,k同型对照
S0B1542反应种属 ?
Hu阳性样本
HeLa纯化方式
Protein G浓度
0.2 mg/ml标记
Pacific Blue性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
MICA (MHC class I polypeptide-related sequence A) and MICB (MHC class I polypeptide-related sequence B) are stress-inducible proteins that belong to the MHC class I family but do not bind to β2-microglobulin or present peptides. They are encoded by genes located within the HLA class I region of chromosome 6. MICA and MICB are expressed on the cell membrane in response to cellular stress or cancer transformation. These proteins act as ligands for the NKG2D receptor, which is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells. The binding of MICA/B to NKG2D can activate these immune cells, leading to cytotoxic responses against stressed or transformed cells. MICA and MICB are highly polymorphic, with over 106 alleles reported for MICA and 25 for MICB. This polymorphism may affect their affinity for NKG2D and, consequently, the activation thresholds of NK and T cells.
流式分析
Flow cytometric analysis of Human MICA expression on HeLa cells. Cells from the HeLa (Human cervix adenocarcinoma epithelial cells) were stained with either Pacific Blue Mouse IgG1, κ Isotype Control (black line histogram) or SDT Pacific Blue Mouse Anti-Human MICA Antibody (red line histogram) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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