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宿主来源
Rat抗原名称
CD199分子别名
C-C chemokine receptor type 9; C-C CKR-9; CC-CKR-9; CCR-9; Chemokine C-C receptor 10; CDw199; Cmkbr10; Ccr9细胞定位
Cell membraneAccession
Q9WUT7克隆号
9B1抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
C57BL/6 mouse thymocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 700性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD199, also known as CCR9, is a member of the G protein-coupled receptor (GPCR) family and functions as a chemokine receptor. It is a ~42 kDa seven-transmembrane protein primarily involved in T cell development in the thymus and gut-associated immune responses. CCR9 is expressed on T lymphocytes and is upregulated in response to retinoic acid, which is produced by dendritic cells, facilitating the migration of T cells to gut-associated tissues. Its specific ligand is CCL25 (TECK), and the interaction between CCR9 and CCL25 plays a crucial role in the recruitment and maturation of thymocytes. Additionally, CCR9 is differentially expressed in T lymphocytes of the small intestine and colon, suggesting its involvement in specialized immune responses within the gastrointestinal tract.
流式分析
Flow cytometric analysis of Mouse CD199 expression on C57BL/6 mouse thymocytes. C57BL/6 mouse thymocytes were stained with Pacific Blue™ Rat Anti-Mouse CD4 and either Alexa Fluor® 700 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Rat Anti-Mouse CD199 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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