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宿主来源
Mouse抗原名称
CD90分子别名
Thy-1 membrane glycoprotein; CDw90; Thy-1 antigen; THY1细胞定位
Cell membraneAccession
P04216克隆号
5E10抗体类型
Mouse mAb抗体同种型
IgG1同型对照
S0B5431反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
Thy-1 or CD90 (Cluster of Differentiation 90) is a ~25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.
流式分析
Flow cytometric analysis of CD90 expression on human PBMCs (human peripheral blood mononuclear cells). Human PBMCs were stained with APC Mouse Anti-Human Lineage Cocktail Antibody and either Biotin Mouse IgG1 Isotype Control (left panel) or SDT Biotin Mouse Anti-Human CD90 Antibody (right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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