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宿主来源
Rabbit克隆号
S-4900抗体类型
Recombinant mAb抗体同种型
IgG纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
FCM
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes labeled with Rabbit IgG Isotype Control antibody at 1/50 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of C57BL/6 mouse splenocytes fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Rabbit IgG Isotype Control antibody at 1/50 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti- Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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