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宿主来源
Rat抗原名称
CD63分子别名
CD63 antigen; Cd63细胞定位
Secreted, Lysosome, Endosome, Cell membraneAccession
P41731克隆号
S-R667抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
C57BL/6 mouse BMDM纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 700性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD63 is a tetraspanin protein involved in various cellular processes such as cell adhesion, migration, and signal transduction. It is predominantly found on the membranes of late endosomes, multivesicular bodies, and exosomes. As a marker protein of exosomes, CD63 plays a crucial role in cell-to-cell communication, including facilitating the interaction between tumor cells and surrounding cells. Additionally, CD63 is associated with intracellular secretory events in cells like eosinophils, where it is involved in degranulation processes. In some cancers, such as hepatocellular carcinoma, CD63-overexpressing tumor-associated macrophages can drive tumor progression by promoting cancer cell proliferation, migration, and invasion.
流式分析
C57BL/6 mouse BMDMs (bone marrow-derived macrophages) were fixed with 4% PFA, permeabilized with 90% methanol. The cells were then stained with Brilliant Violet 421™ Rat Anti-Mouse CD11b Antibody and either Alexa Fluor® 700 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Rat Anti-Mouse CD63 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Flow cytometric analysis of CD63 expression on C57BL/6 mouse BMDMs (bone marrow-derived macrophages). C57BL/6 mouse BMDMs were stained with Brilliant Violet 421™ Rat Anti-Mouse CD11b Antibody and either Alexa Fluor® 700 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Rat Anti-Mouse CD63 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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