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宿主来源
Rat抗原名称
GITR分子别名
Tumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; CD357; Gitr; Tnfrsf18细胞定位
Cell membrane, SecretedAccession
O35714克隆号
DTA-1抗体类型
Rat mAb抗体同种型
Rat IgG2b, λ同型对照
S0B8039反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 0.25μg per million cells in 100μl volume Ms
GITR (glucocorticoid-induced TNF receptor, TNFRSF18/CD357) is a cell-surface receptor of the TNF-receptor superfamily that is constitutively expressed at high levels on regulatory T cells (Tregs) and is rapidly up-regulated on activated CD4⁺/CD8⁺ T cells and NK cells; engagement of GITR by its ligand GITRL (TNFSF18) on antigen-presenting cells delivers a costimulatory signal that activates the NF-κB pathway, enhances IL-2 and IFN-γ production, up-regulates the anti-apoptotic protein Bcl-xL, and thereby amplifies effector T-cell proliferation, cytokine secretion and cytotoxicity while transiently destabilizing FoxP3 and the suppressive function of Tregs, making GITR an attractive target for cancer immunotherapy.
流式分析
Flow cytometric analysis of GITR expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD25 Antibody and either Alexa Fluor® 647 Rat IgG2b, λ Isotype Control (left panel) or SDT Alexa Fluor® 647 Rat Anti-Mouse GITR Antibody (right panel) at 0.5 μl/test. Total viable cells, as determined by Fixable Viability Dye 777 (S0B88805), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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