PE-Cy7 Mouse Anti-Human CD117 (c-kit) Antibody (104D2)
Mast/stem cell growth factor receptor Kit,Piebald trait protein (PBT),Proto-oncogene c-Kit,Tyrosine-protein kinase Kit,p145 c-kit,v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog,SCFR,KIT
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宿主来源
Mouse抗原名称
CD117分子别名
Mast/stem cell growth factor receptor Kit; Piebald trait protein (PBT); Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; SCFR; KIT细胞定位
Cytoplasm, Cell membraneAccession
P10721克隆号
104D2抗体类型
Mouse mAb抗体同种型
IgG1,k同型对照
S0B80046反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
PE-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD117, also known as c-KIT or stem cell factor receptor, is a 145-kDa type III transmembrane receptor tyrosine kinase encoded by the KIT proto-oncogene on chromosome 4q12; upon binding its ligand stem cell factor (SCF) it dimerizes and autophosphorylates, triggering downstream RAS-MAPK, PI3K-AKT, and JAK-STAT cascades that orchestrate melanogenesis, hematopoiesis, mast-cell development, gametogenesis, and interstitial cell of Cajal function, and gain-of-function mutations—most commonly in exons 9, 11, 13, or 17—drive constitutive kinase activity underlying gastrointestinal stromal tumors (GIST), systemic mastocytosis, and subsets of acute myeloid leukemia, making CD117 a key diagnostic immunohistochemical marker and therapeutic target for tyrosine kinase inhibitors such as imatinib, sunitinib, and avapritinib.
流式分析
Flow cytometric analysis of CD117 expression on Human PBMCs. Human PBMCs (peripheral blood mononuclear cells) were stained with Brilliant Violet 421™ Mouse Anti-Human CD34 and either PE-Cy7 Mouse IgG1, κ Isotype Control (left panel) or PE-Cy7 Mouse Anti-Human CD117 Antibody (right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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