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Rat抗原名称
CD45R/B220分子别名
Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen (L-CA); Lymphocyte antigen 5 (Ly-5); T200; CD45; Ly-5; Ptprc细胞定位
Cell membrane, SynapseAccession
P06800克隆号
RA3.3A1/6.1抗体类型
Rat mAb抗体同种型
IgM,k反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes浓度
0.5 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 0.25μg per million cells in 100μl volume Ms
CD45R/B220 is an isoform of the CD45 protein, a member of the protein tyrosine phosphatase (PTP) family, and serves as a critical regulator of T- and B-cell antigen receptor signaling. It is predominantly expressed on the surface of B cells, from early pro-B cells to mature and activated B cells, making it a commonly used pan-B cell marker in mice. Additionally, CD45R/B220 is also found on certain subsets of activated T cells, lymphokine-activated killer cells (LAK), NK cell progenitors, and T cells in mutant mice. This molecule plays a crucial role in lymphocyte development and antigen signaling, and its expression is regulated during different stages of B cell maturation.
流式分析
Flow cytometric analysis of CD45R/B220 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 Antibody and either FITC IgM, k Isotype Control (left panel) or SDT FITC Rat Anti-Mouse CD45R/B220 Antibody (right panel) at 0.5μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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