Alexa Fluor® 488 Mouse Anti-Human CD257 Antibody (S-3021)
Tumor necrosis factor ligand superfamily member 13B,B lymphocyte stimulator (BLyS),B-cell-activating factor,Dendritic cell-derived TNF-like molecule,TNF- and APOL-related leukocyte expressed ligand 1 (TALL-1),BAFF,BLYS,TALL1,TNFSF20,ZTNF4,TNFSF13B
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宿主来源
Mouse抗原名称
CD257分子别名
Tumor necrosis factor ligand superfamily member 13B; B lymphocyte stimulator (BLyS); B-cell-activating factor; Dendritic cell-derived TNF-like molecule; TNF- and APOL-related leukocyte expressed ligand 1 (TALL-1); BAFF; BLYS; TALL1; TNFSF20; ZTNF4; TNFSF13B细胞定位
Cell membraneAccession
Q9Y275克隆号
S-3021抗体类型
Mouse mAb抗体同种型
IgG2a,k同型对照
S0B8029反应种属 ?
Hu阳性样本
U-937纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD257, is a 285-aa type-II transmembrane glycoprotein of the TNF ligand superfamily produced by monocytes, dendritic cells, macrophages and activated T cells that can be cleaved to a soluble homotrimeric cytokine which, via engagement of three receptors—BAFF-R, TACI and BCMA—delivers survival and maturation signals to B cells, orchestrates T-cell co-stimulation, and drives class-switching and antibody production through NF-κB and PI3K/AKT pathways, while excess BAFF triggers B-cell hyperplasia and autoantibody formation, linking its overexpression to systemic lupus erythematosus, Sjögren syndrome, rheumatoid arthritis and multiple myeloma, and making BAFF antagonism (e.g., belimumab) a validated therapeutic strategy.
流式分析
Flow cytometric analysis of CD257 expression on U-937 cells. Cells from the Daudi (Human Burkitt's lymphoma lymphoblast, left) / U-937 (Human histiocytic lymphoma monocyte, right) labelling SDT Alexa Fluor® 488 Mouse Anti-Human CD257 Antibody at 5 μl/test / (red line histogram) compared with Alexa Fluor® 488 Mouse IgG2a, κ (black line histogram) isotype control. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Negative control: Daudi
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