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宿主来源
Rat抗原名称
CD335分子别名
Natural cytotoxicity triggering receptor 1; Activating receptor 1 (mAR-1); Lymphocyte antigen 94; Natural killer cell p46-related protein (NK-p46; NKp46; mNKp46); Ly94; Ncr1细胞定位
Cell membraneAccession
Q8C567克隆号
S-R613抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
PerCP-Cy5.5性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD335, also known as NKp46 or Natural Cytotoxicity Triggering Receptor 1 (NCR1), is a 46 kDa type I transmembrane glycoprotein that belongs to the natural cytotoxicity receptor family. It is primarily expressed on natural killer (NK) cells and plays a crucial role in their activation and cytotoxicity. CD335 can recognize and bind to viral hemagglutinins and heparan sulfate proteoglycans on target cells, triggering NK cell-mediated cytotoxic responses. This receptor is involved in non-MHC-restricted natural cytotoxicity and is essential for the immune system's ability to detect and eliminate infected or transformed cells.
流式分析
Flow cytometric analysis of CD335 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Mouse Anti-Mouse NK1.1 antibody (S0B5488) and either PerCP-Cy5.5 Rat IgG2a, κ Isotype Control (left panel) or SDT PerCP-Cy5.5 Rat Anti-Mouse CD335 Antibody (right panel) at 5 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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