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宿主来源
Mouse抗原名称
CD57分子别名
HNK-1; Leu-7细胞定位
Cell membrane克隆号
S-3011抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azid
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD57 (also designated HNK-1 or Leu-7) is not a protein per se, but a carbohydrate epitope synthesized by the enzymatic action of beta-1,3-glucuronyltransferase 1 (B3GAT1), a type-II membrane protein encoded by the B3GAT1 gene on chromosome 11q25; this enzyme transfers glucuronic acid to terminal N-acetyllactosamine to form the sulfated CD57 determinant on a variety of cell-surface proteins, lipids and chondroitin sulfate proteoglycans, conferring the antigen that marks subsets of terminally differentiated natural killer and CD8+ cytotoxic T lymphocytes, as well as certain neural and muscle cells, and is implicated in neural cell adhesion and the delineation of late-stage lymphocyte maturation.
流式分析
Flow cytometric analysis of CD57 expression on human PBMCs (human peripheral blood mononuclear cells). Human PBMCs were stained with either APC Mouse IgG2a, κ Isotype Control (left panel) or APC Mouse Anti-Human CD57 Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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