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宿主来源
Armenian hamster抗原名称
CD69分子别名
Early activation antigen CD69; Cd69细胞定位
Cell membraneAccession
P37217克隆号
H1.2F3抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Ms阳性样本
Concanavalin A stimulated Mouse splenic leukocytes纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Ms
CD69 is a type II transmembrane C-type lectin protein encoded by the CD69 gene, located on chromosome 12 in humans. It is a homodimer composed of two differentially glycosylated subunits (28–32 kDa), each containing an extracellular C-type lectin domain (CTLD), a transmembrane region, and a short cytoplasmic tail. CD69 is an early activation marker rapidly upregulated on the surface of T cells, B cells, and natural killer (NK) cells after stimulation. Its expression is regulated by transcription factors such as NF-κB, AP-1, and ERG-1. Functionally, CD69 plays a crucial role in lymphocyte proliferation, cytokine production, and retention in lymphoid tissues by interacting with molecules like S1P1 and Myl9/12. It is also implicated in the regulation of immune responses in various inflammatory and autoimmune conditions.
流式分析
Flow cytometric analysis of CD69 expression on unstimulated and stimulated Mouse splenic leukocytes. C57BL/6 Mouse splenocytes were either not stimulated (Left panel) or stimulated overnight (Right panel) with 3 μg/ml Concanavalin A and then stained with either PercP-Cy5.5 Armenian hamster IgG Isotype Control (Black line histogram) and SDT PercP-Cy5.5 Armenian hamster Anti-Mouse CD69 Antibody (Red line histogram) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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