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宿主来源
Armenian hamster抗原名称
CTLA-4 (CD152)分子别名
Cytotoxic T-lymphocyte protein 4; Cd152; Ctla4细胞定位
Cell membraneAccession
P09793克隆号
UC10-4F10-11抗体类型
Recombinant mAb抗体同种型
IgG同型对照
S0B80043反应种属 ?
Ms阳性样本
ConA treated with C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CTLA-4 (Cytotoxic T-Lymphocyte-Associated Protein 4), also known as CD152, is a transmembrane protein expressed on the surface of activated T cells. It plays a role in the negative regulation of the immune response and is one of the key targets in immune checkpoint therapy. CTLA-4 shares the same ligands, B7 molecules (including CD80 and CD86), with CD28; however, unlike CD28, the binding of CTLA-4 to B7 molecules inhibits T cell activation. This inhibitory effect helps prevent excessive immune responses, but in the tumor microenvironment, tumor cells may exploit CTLA-4 to evade immune surveillance and attack.
流式分析
Flow cytometric analysis of Mouse CTLA-4 (CD152) expression on Concanavalin A stimulated C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were either not stimulated (left panel) or stimulated 72h with 2.5μg/ml Concanavalin A (right panel) and then were stained with PE-Cy7 Rat Anti-Mouse CD3 Antibody and SDT FITC Armenian hamster Anti-Mouse CTLA-4 (CD152) Antibody at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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