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宿主来源
Armenian hamster抗原名称
Helios分子别名
Zinc finger protein Helios; HELIOS; ZNFN1A2; IKZF2细胞定位
NucleusAccession
Q9UKS7, P81183克隆号
22F6抗体类型
Recombinant mAb抗体同种型
IgG反应种属 ?
Hu, Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 0.25μg per million cells in 100μl volume Hu, Ms
Helios (encoded by the IKZF2 gene) is a member of the Ikaros family of transcription factors and contains multiple zinc finger domains that facilitate DNA binding and the formation of homodimers or heterodimers. It is predominantly expressed in T cells, regulatory T cells (Tregs), certain B cell subsets, and hematopoietic and epithelial tissues. Helios plays a crucial role in T cell activation, proliferation, and the stability and function of Tregs. Additionally, Helios has been identified as a potential biomarker in autoimmune diseases and may influence tumor development by regulating gene expression and modulating cell differentiation and proliferation.
流式分析
C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Mouse Anti-Human CD4 Antibody and fixed and permeabilized with the eBioscience™ Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent. The cells were then stained with either Alexa Fluor® 488 Armenian hamster IgG Isotype Control (left panel) or SDT Alexa Fluor® 488 Armenian hamster Anti-Mouse/Human Helios Antibody (right panel) at 0.5 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0B88801), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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