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宿主来源
Mouse抗原名称
CD54分子别名
Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; ICAM1细胞定位
MembraneAccession
P05362克隆号
S-3807抗体同种型
IgG2a同型对照
S0B5432反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD54 is an 85–110 kDa transmembrane glycoprotein of the immunoglobulin superfamily that is expressed on endothelial, epithelial and immune cells; upon binding its ligands LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) it serves as the critical adhesion checkpoint that promotes firm leukocyte arrest on the vessel wall, enables subsequent diapedesis into inflamed tissue, and amplifies antigen-specific T-cell activation, thereby positioning it at the mechanistic center of inflammatory, autoimmune and anti-tumor immune responses.
流式分析
Flow cytometric analysis of human CD54 expression on human PBMCs (human peripheral blood mononuclear cells). Human PBMCs were stained with either Biotin Mouse IgG2a Isotype Control (left panel) or SDT Biotin Mouse Anti-Human CD54 Antibody (right panel) at 5 μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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