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宿主来源
Rat抗原名称
Ly-6A/E分子别名
Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Stem cell antigen 1 (SCA-1); T-cell-activating protein (TAP); Ly6; Ly6a细胞定位
Cell membraneAccession
P05533, Q64253克隆号
D7抗体类型
Rat mAb抗体同种型
IgG2a,k同型对照
S0B8044反应种属 ?
Ms阳性样本
ConA treated with C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
Ly-6A/E, also known as Sca-1 (stem cell antigen-1), is an 18-kDa glycosylphosphatidylinositol (GPI)-anchored cell surface protein belonging to the murine Ly-6 multigene family, predominantly expressed on multipotent hematopoietic stem cells in adult mouse bone marrow and fetal liver, and is widely used as a marker for isolating these stem cells; beyond its stem cell association, Ly-6A/E is upregulated on activated T and B lymphocytes and appears to participate in T-cell receptor-mediated activation, myoblast proliferation and differentiation, bone formation, and interferon-induced immune responses, with expression levels varying between mouse strains carrying different Ly-6 haplotypes.
流式分析
Flow cytometric analysis of Mouse Ly-6A/E expression on Concanavalin A treated C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes treated 72h with 2.5μg/ml Concanavalin A were stained with either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 647 Rat Anti-Mouse Ly-6A/E Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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