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宿主来源
Rat抗原名称
CD25分子别名
Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; p55; Il2r; Il2ra细胞定位
MembraneAccession
P01590克隆号
S-R441抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.2 mg/ml标记
APC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Ms
CD25, also known as the interleukin-2 receptor alpha chain (IL2RA), is a type I transmembrane glycoprotein that plays a crucial role in the immune system. It is primarily expressed on the surface of activated T cells and regulatory T cells (Tregs). CD25 is a key component of the high-affinity IL-2 receptor complex, which also includes the IL-2 receptor beta and gamma chains. By itself, CD25 can bind IL-2 with low affinity, but when combined with the beta and gamma chains, it forms a high-affinity receptor that initiates intracellular signaling pathways such as MAPK and JAK/STAT. This receptor complex is essential for the proliferation and activation of T cells, as well as the regulation of immune tolerance by controlling the activity of Tregs. Mutations in the IL2RA gene can lead to deficiencies in IL-2 receptor function, highlighting its importance in maintaining immune homeostasis.
流式分析
Flow cytometric analysis of Mouse CD25 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 488 Rat Anti-Mouse CD4 antibody and either APC Rat IgG2a, κ Isotype Control (Left panel) or SDT APC Rat Anti-Mouse CD25 Antibody (Right panel) at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis.Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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