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宿主来源
Rat抗原名称
CD49f分子别名
Integrin alpha-6; CD49 antigen-like family member F; VLA-6; ITGA6细胞定位
Cell membraneAccession
P23229, Q61739克隆号
GoH3抗体类型
Rat mAb抗体同种型
IgG2a,k同型对照
S0B5537反应种属 ?
Hu, Ms阳性样本
Human PBMC, C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Hu, Ms
CD49f, also known as integrin α6, is a cell surface protein encoded by the ITGA6 gene. It is a type I transmembrane glycoprotein belonging to the α-subfamily of integrins and can form heterodimers with β1 or β4 subunits to function as α6β1 or α6β4 integrins. CD49f is widely expressed in various cell types, including epithelial cells, T cells, monocytes, and platelets, and plays a crucial role in cell adhesion, proliferation, and differentiation. It is also involved in the regulation of cell stemness, particularly in cancer biology, where it marks tumor-initiating cells with high plasticity and immune evasion capabilities. For example, in hepatocellular carcinoma (HCC), CD49f-high tumor cells can recruit neutrophils to create an immunosuppressive microenvironment, contributing to tumor progression and resistance to immune attack.
流式分析
Flow cytometric analysis of CD49f expression on Human PBMCs (human peripheral blood mononuclear cells). Human PBMCs were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 488 Rat Anti-Mouse/Human CD49f Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of CD49f expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti- Mouse CD3 Antibody and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 488 Rat Anti-Mouse/Human CD49f Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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