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宿主来源
Mouse抗原名称
CD4分子别名
T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3细胞定位
Cell membraneAccession
P01730克隆号
S-3179抗体类型
Mouse mAb抗体同种型
IgG1,k同型对照
S0B80046反应种属 ?
Hu阳性样本
Human peripheral blood leukocytes纯化方式
Protein G浓度
0.1 mg/ml标记
PE-Cy7性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 2μl per million cells in 100μl volume Hu
CD4 is a glycoprotein found on the surface of certain immune cells, such as helper T cells, monocytes, macrophages, and dendritic cells. It is a monomeric type I transmembrane molecule composed of four immunoglobulin-like domains (D1 to D4) and encoded by a single gene. As a co-receptor for the T-cell receptor (TCR), CD4 plays a crucial role in the immune response by binding to the MHC class II molecules on antigen-presenting cells, which helps T cells recognize and respond to foreign antigens. The interaction between CD4 and MHC class II molecules also activates downstream signaling pathways, leading to the activation of transcription factors and promoting T cell activation. Additionally, CD4 is significant in HIV infection, as the virus binds to CD4 to enter host cells.
流式分析
Flow cytometric analysis of Human CD4 expression on human peripheral blood leukocytes. Human peripheral blood leukocytes were stained with Alexa Fluor® 647 Mouse Anti-Human CD3 Antibody and either PE-Cy7 Mouse IgG1, κ Isotype Control (left panel) or SDT PE-Cy7 Mouse Anti- Human CD4 Antibody (right panel) at 2 μl/test treated with True-Stain Monocyte Blocker™. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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