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宿主来源
Mouse克隆号
S-5115抗体类型
Mouse mAb抗体同种型
IgG2a,k纯化方式
Protein A浓度
0.2 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
ICFCM
FCM
Isotype control antibodies, to estimate the nonspecific binding of target. Use at concentrations comparable to those of the specific antibody of interest.
流式分析
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cells) labeled with Mouse IgG2a, κ Isotype Control antibody at 1/20 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cells) fixed with 4% paraformaldehyde and permeabilized with 0.1% Tween. Cells were labeled with Mouse IgG2a, κ Isotype Control antibody at 1/20 dilution (1 μg) / (right panel) compared with an unlable Control / (left panel). Goat Anti-Mouse IgG Alexa Fluor® 488 was used as the secondary antibody. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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