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宿主来源
Rat抗原名称
CD205分子别名
Lymphocyte antigen 75; Ly-75; DEC-205; Cd205; Ly75细胞定位
MembraneAccession
Q60767克隆号
NLDC-145抗体类型
Rat mAb抗体同种型
IgG2a,k同型对照
S0B5537反应种属 ?
Ms阳性样本
C57BL/6 mouse bone marrow纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD205, also known as DEC-205, is a type C lectin receptor and a type I membrane glycoprotein belonging to the mannose receptor family. It is highly expressed on dendritic cells (DCs), thymic epithelial cells, and certain epithelial cells. CD205 plays a crucial role in antigen uptake, processing, and presentation, facilitating the induction of antigen-specific T cell immunity. This receptor is involved in endocytosis, capturing antigens from the extracellular space and transporting them to specialized compartments for processing. Its high efficiency in antigen presentation makes it a valuable target in immunotherapy, particularly for developing vaccines against viruses and tumors. Additionally, CD205 has been found to recognize apoptotic and necrotic cells in an acidic environment through its interaction with keratin.
流式分析
Flow cytometric analysis of Mouse CD205 expression on BALB/c mouse bone marrow. BALB/c mouse bone marrow was stained with Pacific Blue™ Rat Anti-Mouse Ly-6G/Ly-6C Antibody and either Alexa Fluor® 488 Rat IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 488 Rat Anti-Mouse CD205 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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