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宿主来源
Mouse抗原名称
CD272 (BTLA)分子别名
B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; Btla细胞定位
Cell membraneAccession
Q7TSA3克隆号
S-3831抗体类型
Mouse mAb抗体同种型
IgG1同型对照
S0B1519反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD272 (BTLA) is a ~34–43 kDa type-I transmembrane glycoprotein of the CD28/CTLA-4 Ig superfamily that is constitutively expressed on T, B, NK and dendritic cells, where it delivers inhibitory signals through two ITIMs and an ITSM in its cytoplasmic tail after binding its ligand HVEM (CD270), thereby attenuating T- and B-cell proliferation, cytokine production and cytolytic activity, recruiting SHP-1/SHP-2 phosphatases to maintain immune homeostasis and self-tolerance, and acting in concert with PD-1 and CTLA-4 as an immune-checkpoint axis whose down-regulation on tumor or autoreactive lymphocytes promotes immune evasion or autoimmune pathologies such as SLE.
流式分析
Flow cytometric analysis of Mouse CD272 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Pacific Blue Rat Anti-Mouse CD45R/B220 Antibody and either Alexa Fluor® 488 Mouse IgG1 Isotype Control (left panel) or SDT Alexa Fluor® 488 Mouse Anti-Mouse CD272 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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