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宿主来源
Rat抗原名称
IL-10分子别名
Interleukin-10; IL-10; Cytokine synthesis inhibitory factor (CSIF); IL10细胞定位
SecretedAccession
P22301克隆号
S-3081抗体类型
Rat mAb抗体同种型
IgG2a,k同型对照
S0B5537反应种属 ?
Hu阳性样本
Th2 polarized human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 5μl per million cells in 100μl volume Hu
Interleukin-10 (IL-10) is a cytokine that plays a crucial role in regulating immune responses. It is primarily produced by various immune cells, including monocytes, macrophages, and regulatory T cells. IL-10 has potent anti-inflammatory properties, as it inhibits the production of pro-inflammatory cytokines such as IL-1, TNF-α, and IFN-γ from activated macrophages and T helper 1 cells. By doing so, it helps to prevent excessive inflammation and tissue damage. Additionally, IL-10 promotes the differentiation of regulatory T cells and enhances the function of B cells, contributing to the maintenance of immune homeostasis. Dysregulation of IL-10 production has been implicated in various diseases, including autoimmune disorders and certain types of cancer, highlighting its importance in both health and disease.
流式分析
Flow cytometric analysis of IL-10 expression in human PBMC polarized with Th2 Polarization Kit, Human (UA090018). Th2 polarized human PBMC were harvested and restimulated for 5 hr with PMA and Ionomycin in the presence of Brefeldin A (right Panel) or unstimulated (left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with Phycoerythrin Mouse Anti-Human CD4 Antibody and SDT Alexa Fluor® 488 Rat Anti-Human IL-10 Antibody at 5 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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