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宿主来源
Rat抗原名称
CD134分子别名
Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; Ox40; Txgp1; Tnfrsf4细胞定位
MembraneAccession
P47741克隆号
OX-86抗体类型
Rat mAb抗体同种型
IgG1,k反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes treated 72h with 2.5 μg/ml Concanavalin A纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD134, also known as TNFRSF4 or OX40, is a member of the tumor necrosis factor receptor superfamily. It is a co-stimulatory molecule primarily expressed on activated CD4+ and CD8+ T cells, as well as other immune cells such as natural killer (NK) cells and dendritic cells. Structurally, CD134 contains four TNFR-Cys repeats and interacts with its ligand, OX40L (TNFSF4), to activate downstream signaling pathways, including the NF-κB pathway through adaptor proteins like TRAF2 and TRAF5. This interaction promotes T cell proliferation, cytokine production, and enhances the survival of CD8+ T cells, playing a crucial role in immune responses. Additionally, CD134 has been implicated in various diseases, including cancer and autoimmune conditions, making it a target for immunotherapy and other therapeutic interventions.
流式分析
Flow cytometric analysis of C57BL/6 mouse splenocytes treated for 72 h with 2.5 μg/ml Concanavalin A (right panel) or untreated (left panel). Cells were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 Antibody and SDT Alexa Fluor® 488 Rat Anti-Mouse CD134 Antibody at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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