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Mouse抗原名称
MHC Class I (H-2Kb)分子别名
H-2 class I histocompatibility antigen, K-B alpha chain; H-2K(B); H2-K; H2-K1细胞定位
MembraneAccession
P01901克隆号
AF6-88.5抗体类型
Mouse mAb抗体同种型
IgG2a,k同型对照
S0B80171反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein A浓度
0.5 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 0.25μg per million cells in 100μl volume Ms
MHC Class I (H-2Kb) is a 45 kDa type I transmembrane glycoprotein encoded within the mouse MHC locus on chromosome 17, functioning as the murine ortholog of human HLA-A; it folds in the endoplasmic reticulum with a 12 kDa β2-microglobulin light chain to create a peptide-binding groove that preferentially accommodates 8–10-mer peptides bearing hydrophobic C-termini, and once loaded it is transported to the cell surface where it surveys CD8⁺ cytotoxic T lymphocytes for the presence of viral, tumor or self-peptides, thereby orchestrating rapid immune elimination of aberrant cells while maintaining tolerance through engagement of inhibitory receptors such as Ly49 and KIR.
流式分析
Flow cytometric analysis of H-2Kb expression on C57BL/6 mouse splenocytes. Cells from the C57BL/6 mouse splenocytes (right) or BALB/c mouse splenocytes (left) were stained with either Alexa Fluor® 488 Mouse IgG2a, κ Isotype Control (black line histogram) or SDT Alexa Fluor® 488 Mouse Anti-Mouse H-2Kb Antibody (red line histogram) at 0.5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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