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宿主来源
Mouse抗原名称
CD11b/c分子别名
Integrin beta 2 alpha subunit; Itgam; Integrin subunit alpha X; Itgax细胞定位
MembraneAccession
Q9JI30, D3ZWZ1克隆号
OX-42抗体类型
Mouse mAb抗体同种型
IgG2a,k反应种属 ?
Rt阳性样本
SD rat splenocytes纯化方式
Protein A浓度
0.5 mg/ml标记
Alexa Fluor® 700性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Rt
CD11b and CD11c are two distinct integrin proteins that play crucial roles in the immune system. CD11b, also known as integrin alpha-M, is primarily expressed on myeloid cells such as macrophages and dendritic cells. It is involved in cell adhesion, migration, and immune cell activation. For example, CD11b ligation promotes pro-inflammatory macrophage signaling and regulates macrophage polarization. CD11c, on the other hand, is mainly found on dendritic cells and is associated with antigen presentation and T cell activation. CD11c+ CD11b+ dendritic cells have been shown to play an important role in the induction of T regulatory cells and the suppression of autoimmune diseases.
流式分析
Flow cytometric analysis of CD11b/c expression on SD rat splenocytes. SD rat splenocytes were stained with Phycoerythrin Mouse Anti-Rat CD3 Antibody and either Alexa Fluor® 700 Mouse IgG2a, κ Isotype Control (left panel) or SDT Alexa Fluor® 700 Mouse Anti-Rat CD11b/c Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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