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宿主来源
Rat抗原名称
IL-2分子别名
Interleukin-2; T-cell growth factor (TCGF); Il-2; Il2细胞定位
SecretedAccession
P04351克隆号
JES6-5H4抗体类型
Rat mAb抗体同种型
IgG2b,k同型对照
S0B8039反应种属 ?
Ms阳性样本
PMA stimulated C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1μg per million cells in 100μl volume Ms
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in the immune system. It is primarily produced by activated T cells and has multiple functions. IL-2 promotes the proliferation and differentiation of T cells, enhancing their ability to fight infections and tumors. It also stimulates the growth and activity of natural killer (NK) cells, which are important for immune defense. Additionally, IL-2 can regulate the activity of regulatory T cells (Tregs), helping to maintain immune tolerance and prevent autoimmune diseases. In clinical settings, IL-2 has been used to treat certain cancers by boosting the immune response, although its use is limited due to potential side effects. Its discovery and research have significantly advanced our understanding of the immune system and provided new strategies for immunotherapy.
流式分析
Flow cytometric analysis of IL-2 expression in C57BL/6 mouse splenocytes stimulated with PMA and Ionomycin in the presence of Brefeldin A for 6hr (right Panel) or unstimulated (left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with SDT Alexa Fluor® 647 Rat Anti-Mouse IL-2 Antibody at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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