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Rabbit抗原名称
CD40分子别名
Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; TNFRSF5免疫原
Recombinant Protein细胞定位
Secreted, Cell membraneAccession
P25942克隆号
S-145-45抗体类型
Recombinant mAb抗体同种型
IgG同型对照
S0B8021反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD40, also known as TNFRSF5 or Bp50, is a 48 kDa type I membrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is widely expressed on various immune cells, including B cells, dendritic cells, and macrophages. The interaction between CD40 and its ligand, CD40L, is crucial for immune cell activation and regulation. Structurally, CD40 consists of a 62 amino acid cytoplasmic tail, a 22 amino acid transmembrane domain, and a 173 amino acid extracellular domain. This co-stimulatory protein plays a key role in B cell activation, proliferation, and differentiation, and is also involved in immune responses against infections and tumors. Due to its significant role in the immune system, CD40 has become an important therapeutic target, with various agonistic and antagonistic antibodies being developed to modulate its activity.
流式分析
Flow cytometric analysis of CD40 expression on human PBMC. Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD19 and either Alexa Fluor® 647 Rabbit IgG Isotype Control (left panel) or SDT Alexa Fluor® 647 Rabbit Anti-Human CD40 Antibody (right panel) at 5μl/test. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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