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宿主来源
Rat抗原名称
CD144分子别名
Cadherin-5; Vascular endothelial cadherin (VE-cadherin); Cdh5细胞定位
Cytoplasm, Cell membraneAccession
P55284克隆号
S-R719抗体类型
Rat mAb抗体同种型
IgG1,k反应种属 ?
Rt阳性样本
bEnd.3纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Ms
CD144, also known as VE-cadherin or cadherin-5, is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail, expressed almost exclusively at the adherens junctions of endothelial cells in blood and lymphatic vessels, where it mediates homophilic adhesion, controls endothelial permeability, maintains vascular integrity, promotes endothelial quiescence and tubulogenesis, and is indispensable for embryonic vascular development since its genetic deletion or truncation causes mid-gestational lethality due to vessel disassembly and hemorrhage.
流式分析
Flow cytometric analysis of Mouse CD144 expression on bEnd.3 cells. Cells from the bEnd.3 (Mouse brain endothelioma, Right) or NIH/3T3 (Mouse embryonic fibroblast, Left) was stained with Alexa Fluor® 647 Rat Anti-Mouse CD144 Antibody Rat IgG1 κ Isotype Control (Black line histogram) and SDT Alexa Fluor® 647 Rat Anti-Mouse CD144 Antibody (Red line histogram) at 1.25 μl/test. ow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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