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宿主来源
Mouse抗原名称
CD25分子别名
Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; IL2RA细胞定位
MembraneAccession
P01589克隆号
3G10抗体类型
Mouse mAb抗体同种型
IgG1同型对照
S0B5964反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD25, also known as IL2RA, is the alpha chain of the interleukin-2 receptor, a heterotrimeric protein complex that also includes beta (IL2RB) and the common gamma chain (IL2RG). It is a membranous glycoprotein primarily expressed on activated T lymphocytes and plays a crucial role in immune homeostasis by interacting with interleukin-2, a pleiotropic cytokine. CD25 is involved in the proliferation of T and B lymphocytes and is essential for the assembly of the high-affinity interleukin-2 receptor. In addition to its role in the immune system, CD25 is also expressed on certain non-immune cells and has been identified as a potential therapeutic target in cancers.
流式分析
Flow cytometric analysis of CD25 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD4 Antibody and either Alexa Fluor® 647 Mouse IgG1 Isotype Control (left panel) or SDT Alexa Fluor® 647 Mouse Anti-Human CD25 Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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