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宿主来源
Rat抗原名称
Ki67分子别名
Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog (Antigen KI-67 homolog; Antigen Ki67 homolog); Mki67细胞定位
NucleusAccession
E9PVX6克隆号
S-R595抗体类型
Rat mAb抗体同种型
IgG2a,k同型对照
S0B5537反应种属 ?
Ms阳性样本
ConA treated C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1μg per million cells in 100μl volume Ms
Ki67 protein is a non-histone nuclear protein encoded by the MKI67 gene, widely used as a proliferation marker in pathology. It is expressed during all active phases of the cell cycle—G1, S, G2, and M—but is absent in quiescent (G0) cells. Ki67 has multiple molecular functions, including forming the perichromosomal layer to prevent chromosome aggregation during mitosis and organizing heterochromatin. It also facilitates chromosome attachment to the mitotic spindle and individual chromosome mobility. In addition to its role in cell division, Ki67 is a valuable prognostic and predictive indicator in cancer diagnosis, with its expression levels correlating with tumor cell proliferation rates.
流式分析
Flow cytometric analysis of Ki-67 expression on ConA treated C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes treated 72h with ConA were stained with Alexa Fluor® 488 Rat IgG2a, k Isotype Control (left panel) or SDT Alexa Fluor® 488 Rat Anti-Mouse Ki-67 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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