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宿主来源
Rat抗原名称
IL-10分子别名
Interleukin-10; Cytokine synthesis inhibitory factor (CSIF); Il-10; Il10细胞定位
SecretedAccession
P18893克隆号
S-R643抗体类型
Rat mAb抗体同种型
IgG2b,k同型对照
S0B5536反应种属 ?
Ms阳性样本
Th2 polarized C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
ICFCM
稀释度
应用 稀释度 推荐种属 ICFCM 1μg per million cells in 100μl volume Ms
Interleukin-10 (IL-10) is an 18 kDa anti-inflammatory cytokine composed of 160 amino acids folded into two intrachain disulfide-linked α-helical dimers that signals through the heterotetrameric IL-10R1/IL-10R2 receptor complex to activate JAK1/TYK2-mediated STAT3 phosphorylation, thereby down-regulating major histocompatibility class II, co-stimulatory molecules and pro-inflammatory cytokines such as TNF-α, IL-1, IL-6, IL-8, IL-12 and IFN-γ, while simultaneously up-regulating IL-1RA and soluble TNF receptors, ultimately exerting potent immunosuppressive and tissue-protective functions in both innate and adaptive immunity.
流式分析
Flow cytometric analysis of IL-10 expression in C57BL/6 mouse splenocytes CD4+ T cells stimulated with Invivo anti-mouse CD3ε Recombinant mAb (S0B0661, 10 μg/ml for palate coating) and NA/LE Syrian Hamster anti-mouse CD28 mAb (S0B0003E, 2 μg/ml for palate coating) alone with IL-2(10 ng/ml for palate coating) and IL-4(15 ng/ml for palate coating). The cells were then harvested and restimulated for 6 hr with PMA and Ionomycin in the presence of Monensin (right Panel) or unstimulated (left Panel).
The cells were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with SDT Alexa Fluor® 488 Rat Anti-Mouse IL-10 Antibody at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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