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宿主来源
Rabbit抗原名称
CD4分子别名
T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3细胞定位
Cell membrane, Endoplasmic reticulumAccession
P01730克隆号
13B8.2抗体同种型
IgG同型对照
S0B0161反应种属 ?
Hu阳性样本
human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD4 is a glycoprotein found on the surface of certain immune cells, such as helper T cells, monocytes, macrophages, and dendritic cells. It is a monomeric type I transmembrane molecule composed of four immunoglobulin-like domains (D1 to D4) and encoded by a single gene. As a co-receptor for the T-cell receptor (TCR), CD4 plays a crucial role in the immune response by binding to the MHC class II molecules on antigen-presenting cells, which helps T cells recognize and respond to foreign antigens. The interaction between CD4 and MHC class II molecules also activates downstream signaling pathways, leading to the activation of transcription factors and promoting T cell activation. Additionally, CD4 is significant in HIV infection, as the virus binds to CD4 to enter host cells.
流式分析
Flow cytometric analysis of Human CD4 expression on human PBMC (human peripheral blood mononuclear cell). Human PBMC were stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either Alexa Fluor® 488 Rabbit IgG Isotype Control (left panel) or SDT Alexa Fluor® 488 Rabbit Anti-Human CD4 Antibody (right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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