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Mouse抗原名称
CD107b/LAMP2分子别名
Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; LGP-96细胞定位
Cell membraneAccession
P13473克隆号
H4B4抗体类型
Mouse mAb抗体同种型
IgG1,k同型对照
S0B1519反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 ICFCM 5μl per million cells in 100μl volume Hu
CD107b, also known as LAMP2 (Lysosome-Associated Membrane Protein 2), is a heavily glycosylated type I transmembrane protein that constitutes a major component of the lysosomal membrane, where it plays critical roles in maintaining lysosomal integrity, autophagy, and chaperone-mediated autophagy by acting as a receptor for chaperone–substrate complexes on the lysosomal surface.
流式分析
Human PBMC (human peripheral blood mononuclear cells) were fixed with 4% PFA and permeabilization with 0.1% Tween. Cells were then intracellularly stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and either Alexa Fluor® 488 Mouse IgG1, k Isotype Control (left panel) or SDT Alexa Fluor® 488 Mouse Anti-Human CD107b/LAMP2 Antibody (right panel) at 5μl/test. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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