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Mouse抗原名称
B7-H4分子别名
V-set domain-containing T-cell activation inhibitor 1; B7 homolog 4 (B7-H4); B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; T-cell costimulatory molecule B7x; B7H4; VTCN1细胞定位
Cell membraneAccession
Q7Z7D3克隆号
S-3024抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
SK-BR-3纯化方式
Protein G浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
B7-H4, also known as VTCN1 or B7x, is a ~50 kDa type-I trans-membrane glycoprotein of the B7 immunoglobulin superfamily that consists of an extracellular IgV-IgC domain pair, a single trans-membrane segment and a short cytoplasmic tail; it is expressed inducibly on antigen-presenting cells and constitutively on many epithelial cancers, where it acts as an immune-checkpoint ligand by binding an as-yet-unidentified receptor on activated CD4⁺ and CD8⁺ T cells, thereby arresting the cell cycle in G0/G1, suppressing IL-2 production and proliferation, and enabling tumours to evade immunity, making it an emerging target for cancer immunotherapy.
流式分析
Flow cytometric analysis of B7-H4 expression on SK-BR-3 cells. Cells from the SK-BR-3 (Human breast adenocarcinoma epithelial cell, right) or HeLa (Human cervix adenocarcinoma epithelial cell, left) were stained with either FITC Mouse IgG1, κ Isotype Control (black line histogram) or SDT FITC Mouse Anti-Human B7-H4 Antibody (red line histogram) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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