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Mouse抗原名称
CD144分子别名
Cadherin-5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CDH5细胞定位
Cell membrane, Cell junctionAccession
P33151克隆号
S-3026抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
HUVEC纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD144, also termed VE-cadherin or cadherin-5, is a 140 kDa calcium-dependent type-II classical cadherin expressed almost exclusively on vascular and lymphatic endothelial cells, where it forms the core of adherens junctions by mediating homophilic trans-interactions between neighboring cells and anchoring to the actin cytoskeleton via catenins (β-, γ-, and p120-catenin), thereby controlling endothelial barrier integrity, vascular permeability, leukocyte extravasation, and embryonic angiogenesis, while also modulating VEGF receptor signaling, cell polarity, and lumen formation .
流式分析
Flow cytometric analysis of CD144 expression on HUVEC (human umbilical vein endothelial cell). Cells from HUVEC were stained with either Biotin Mouse IgG1, κ Isotype Control (black line histogram) or SDT Biotin Mouse Anti-Human CD144 Antibody (red line histogram) at 5 μl/test followed by Sav-iFluor 488, cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabeled control. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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