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Mouse抗原名称
CD54分子别名
Intercellular adhesion molecule 1; ICAM-1; Major group rhinovirus receptor; ICAM1细胞定位
MembraneAccession
P05362克隆号
S-3807抗体类型
Mouse mAb抗体同种型
IgG2a反应种属 ?
Hu阳性样本
Human PBMC纯化方式
Protein A浓度
0.1 mg/ml标记
Alexa Fluor® 647性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 2 μl per million cells in 100μl volume Hu
ICAM-1/CD54 is an 85–110 kDa transmembrane glycoprotein of the immunoglobulin superfamily that is expressed on endothelial, epithelial and immune cells; upon binding its ligands LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) it serves as the critical adhesion checkpoint that promotes firm leukocyte arrest on the vessel wall, enables subsequent diapedesis into inflamed tissue, and amplifies antigen-specific T-cell activation, thereby positioning it at the mechanistic center of inflammatory, autoimmune and anti-tumor immune responses.
流式分析
Flow cytometric analysis of CD54 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Alexa Fluor® 647 Mouse IgG2a Isotype Control (left panel) or SDT Alexa Fluor® 647 Mouse Anti-Human CD54 Antibody (right panel) at 2 μl/test. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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