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Mouse抗原名称
CD144分子别名
Cadherin-5; 7B4 antigen; Vascular endothelial cadherin (VE-cadherin); CDH5细胞定位
Cytoplasm, Cell membraneAccession
P33151克隆号
S-3026抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
HUVEC纯化方式
Protein G浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
CD144, also termed VE-cadherin or cadherin-5, is a 140 kDa calcium-dependent type-II classical cadherin expressed almost exclusively on vascular and lymphatic endothelial cells, where it forms the core of adherens junctions by mediating homophilic trans-interactions between neighboring cells and anchoring to the actin cytoskeleton via catenins (β-, γ-, and p120-catenin), thereby controlling endothelial barrier integrity, vascular permeability, leukocyte extravasation, and embryonic angiogenesis, while also modulating VEGF receptor signaling, cell polarity, and lumen formation .
流式分析
Flow cytometric analysis of CD144 expression on HUVEC (human umbilical vein endothelial cell). Cells from HUVEC were stained with either Alexa Fluor® 488 Mouse IgG1, κ Isotype Control (black line histogram) or SDT Alexa Fluor® 488 Mouse Anti-Human CD144 Antibody (red line histogram) at 5 μl/test. Cells without incubation with primary antibody and secondary antibody (blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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