分享产品介绍 评论(0)
宿主来源
Mouse抗原名称
CD3分子别名
T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; Cd3g细胞定位
Cell membraneAccession
Q64159, P19377克隆号
1F4抗体类型
Mouse mAb抗体同种型
IgG2b反应种属 ?
Rt阳性样本
SD Rat splenocytes纯化方式
Protein A浓度
0.2 mg/ml标记
Alexa Fluor® 488性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Rt
CD3 is a multimeric protein complex that serves as a crucial T-cell co-receptor, composed of four distinct chains (CD3γ, δ, ε, ζ) that non-covalently associate with the T-cell receptor (TCR), where its cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) become phosphorylated upon antigen recognition to initiate downstream signaling cascades leading to T-cell activation, differentiation, and effector functions, making it an essential component of adaptive immunity and a valuable target for therapeutic interventions such as the immunosuppressive agent muromonab-CD3 and emerging bispecific antibodies in oncology.
流式分析
Flow cytometric analysis of Rat CD3 expression on SD Rat splenocytes. SD Rat splenocytes were stained with either Alexa Fluor® 488 Mouse IgG2b Isotype Control (Black line histogram) or SDT Alexa Fluor® 488 Mouse Anti-Rat CD3 Antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
评论(0)