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宿主来源
Mouse抗原名称
CD357分子别名
Tumor necrosis factor receptor superfamily member 18; Activation-inducible TNFR family receptor; Glucocorticoid-induced TNFR-related protein; AITR; GITR; TNFRSF18细胞定位
Cell membraneAccession
Q9Y5U5克隆号
S-3126抗体类型
Mouse mAb抗体同种型
IgG2b,k反应种属 ?
Hu阳性样本
PHA-treated human PBMC纯化方式
Protein A浓度
0.2 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1.25μl per million cells in 100μl volume Hu
CD357, synonymous with GITR (glucocorticoid-induced TNFR-related protein) and TNFRSF18, is a type I transmembrane receptor of the tumor necrosis factor receptor superfamily that is constitutively expressed on CD25⁺CD4⁺ regulatory T cells and up-regulated on activated CD4⁺ and CD8⁺ T cells, NK cells, APCs and many tumors; upon binding its ligand TNFSF18 it recruits TRAF2 and TRAF5, triggering NF-κB and MAPK signaling that enhances effector T-cell proliferation, cytokine production and resistance to suppression by Tregs, positioning CD357 as a promising co-stimulatory target in cancer immunotherapy .
流式分析
Flow cytometric analysis of human CD357 expression on PHA activated human PBMC (human peripheral blood mononuclear cells). Human PBMC were untreated (left panel) or cultured for 2 days in medium with 10μg/ml PHA (right Panel) were then stained with Brilliant Violet 421™ Mouse Anti-Human CD3 Antibody and SDT FITC Mouse Anti-Human CD357 Antibody at 1.25 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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