分享产品介绍 评论(0)
宿主来源
Rat抗原名称
CD182分子别名
C-X-C chemokine receptor type 2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B (IL-8R B); Cmkar2; Gpcr16; Il8rb; Cxcr2细胞定位
Cell membraneAccession
P35343克隆号
S-R718抗体类型
Rat mAb抗体同种型
IgG2a,k反应种属 ?
Ms阳性样本
C57BL/6 mouse bone marrow纯化方式
Protein G浓度
0.5 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD182, also known as CXCR2 or IL-8 receptor beta, is a 67-70 kDa member of the G-protein-coupled receptor family with seven transmembrane regions. It is expressed on various cell types, including neutrophils, natural killer cells, T cell subsets, monocytes, endothelial cells, and others. CD182 plays a crucial role in mediating chemotaxis, cell activation, proliferation, and angiogenesis. It binds to chemokines such as IL-8, NAP-2, GCP-2, and GRO-alpha, -beta, and -gamma, which induce these biological effects. This receptor is also involved in neutrophil migration to sites of inflammation and is essential for the positioning of oligodendrocyte precursors in the developing spinal cord.
流式分析
Flow cytometric analysis of mouse CD182 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow were stained with Pacific blue™ Rat Anti-Mouse Ly6G/Ly6C Antibody and either Biotin Rat IgG2a, k Isotype Control (left panel) or SDT Biotin Rat Anti-Mouse CD182 Antibody (right panel) at 2 μl/test followed by Sav-PE. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
评论(0)