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宿主来源
Rat抗原名称
CD96分子别名
T-cell surface protein tactile; Cell surface antigen CD96; T cell-activated increased late expression protein; Cd96细胞定位
MembraneAccession
Q3U0X8克隆号
3.3抗体类型
Rat mAb抗体同种型
IgG1,λ反应种属 ?
Ms阳性样本
C57BL/6 mouse splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 30
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Ms
CD96, also known as TACTILE (T cell activation, increased late expression), is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, encoded by the CD96 gene. It is primarily expressed on T cells and natural killer (NK) cells, and is involved in immune regulation. CD96 shares sequence similarity with CD226 (DNAM-1) and competes with it for binding to CD155, its main ligand. This interaction can inhibit NK cell function and cytokine responses, making CD96 a potential target for cancer immunotherapy. Research has shown that blocking CD96 can enhance antitumor immunity, particularly when combined with other treatments such as anti-PD-1 or anti-CTLA-4 therapies. Additionally, CD96 expression is associated with immune infiltration and poor clinical outcomes in some cancers, further highlighting its potential as a therapeutic target.
流式分析
Flow cytometric analysis of mouse CD96 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Brilliant Violet 421™ Rat Anti-Mouse CD3 Antibody and either Biotin Rat IgG1, λ Isotype Control (left panel) or SDT Biotin Rat Anti- Mouse CD96 Antibody (right panel) at 2 μl/test followed by Sav-PE. Total viable cells, as determined by Fixable Viability Dye 515 (S0D0013), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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