Pacific Blue Rat Anti-Mouse/Human CD44 Antibody (IM7)
CDw44,Epican,Extracellular matrix receptor III (ECMR-III),GP90 lymphocyte homing/adhesion receptor,HUTCH-I,Heparan sulfate proteoglycan,Hermes antigen,Hyaluronate receptor,Phagocytic glycoprotein 1 (PGP-1),Phagocytic glycoprotein I (PGP-I),LHR,MDU2,MDU3,MIC4
分享产品介绍 评论(0)
宿主来源
Rat抗原名称
CD44分子别名
CDw44; Epican; Extracellular matrix receptor III (ECMR-III); GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1 (PGP-1); Phagocytic glycoprotein I (PGP-I); LHR; MDU2; MDU3; MIC4细胞定位
Cell membraneAccession
P16070, P15379克隆号
IM7抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Hu, Ms阳性样本
C57BL/6 mouse bone marrow, human peripheral blood cells纯化方式
Protein G浓度
0.5 mg/ml标记
Pacific Blue性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 30
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Hu, Ms
CD44 is a widely expressed transmembrane glycoprotein on the cell surface, belonging to the adhesion molecule family. It primarily functions as a receptor for hyaluronic acid (HA) and mediates cell-cell and cell-matrix interactions. Its structure includes variant exon-spliced isoforms (e.g., CD44v) and a constant region (CD44s), with post-translational modifications such as glycosylation generating multiple variants. CD44 plays crucial roles in physiological and pathological processes, including inflammation, wound healing, lymphocyte homing, and tumor metastasis. It is highly expressed in cancer stem cells (CSCs) and is associated with tumor progression, drug resistance, and poor prognosis. Key signaling pathways involving CD44 include PI3K/AKT and RAS/MAPK, making it a potential therapeutic target in cancer and stem cell research.
流式分析
Flow cytometric analysis of CD44 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow were stained with Pacific Blue Rat IgG2b, κ Isotype Control (left panel) or SDT Pacific Blue Rat Anti-Mouse/Human CD44 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of CD44 expression on human peripheral blood cells. Human peripheral blood cells were stained with Pacific Blue Rat IgG2b, κ Isotype Control (left panel) or SDT Pacific Blue Rat Anti-Mouse/Human CD44 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
评论(0)