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Mouse抗原名称
Siglec-8分子别名
Sialic acid-binding Ig-like lectin 8; Siglec-8; Sialoadhesin family member 2 (SAF-2); SAF2; SIGLEC8细胞定位
MembraneAccession
Q9NYZ4克隆号
S-4479抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Hu阳性样本
Human peripheral blood cells纯化方式
Protein G浓度
0.2 mg/ml标记
Biotin性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 5μl per million cells in 100μl volume Hu
Siglec-8 is a CD33-related cell-surface lectin selectively expressed on human eosinophils and, at lower density, on mast cells and basophils; it carries an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif that, upon engagement by sialylated glycan ligands, recruit SHP-1/2 phosphatases to deliver a potent inhibitory signal that suppresses FcεRI- or cytokine-driven activation, survival and mediator release, thereby serving as a key “off-switch” for allergic inflammation and making it an attractive therapeutic target for eosinophilic asthma, chronic urticaria and other IgE-mediated diseases.
流式分析
Flow cytometric analysis of human Siglec-8 expression on human peripheral blood cells. Human peripheral blood cells were stained with PE Mouse Anti-Human CD193 Antibody and either Biotin Mouse IgG1, κ Isotype Control (left panel) or SDT Biotin Mouse Anti-Human Siglec-8 Antibody (right panel) at 5 μl/test followed by Sav-APC. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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