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宿主来源
Rat抗原名称
CD44分子别名
CD44 antigen; CDw44; Epican; Extracellular matrix receptor III (ECMR-III); GP90 lymphocyte homing/adhesion receptor; HUTCH-I; LHR; MDU2; MDU3; MIC4细胞定位
Cell membraneAccession
P16070, P15379克隆号
IM7抗体类型
Rat mAb抗体同种型
IgG2b,k反应种属 ?
Hu, Ms阳性样本
C57BL/6 mouse bone marrow, Human peripheral blood cells纯化方式
Protein G浓度
0.5 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Hu, Ms
CD44 is a multifunctional transmembrane glycoprotein that functions as a major adhesion molecule for hyaluronan and other extracellular matrix components, playing critical roles in cell migration, proliferation, differentiation, and survival; it is expressed in numerous isoforms generated through alternative splicing, with its standard form (CD44s) and variant isoforms (CD44v) exhibiting distinct tissue distributions and functional properties, and its dysregulated expression has been implicated in various pathological processes including inflammation, autoimmune diseases, and cancer progression where it contributes to tumor cell invasion, metastasis, and resistance to apoptosis.
流式分析
Flow cytometric analysis of CD44 expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow were stained with FITC Rat IgG2b, κ Isotype Control (left panel) or SDT FITC Rat Anti-Mouse/Human CD44 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
Flow cytometric analysis of CD44 expression on human peripheral blood cells. Human peripheral blood cells were stained with FITC Rat IgG2b, κ Isotype Control (left panel) or SDT FITC Rat Anti-Mouse/Human CD44 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 662 (S0D0016), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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