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宿主来源
Mouse抗原名称
CD47分子别名
Leukocyte surface antigen CD47; Integrin-associated protein (IAP); Cd47细胞定位
Cell membraneAccession
P97829克隆号
OX-101抗体类型
Mouse mAb抗体同种型
IgG1,k反应种属 ?
Rt阳性样本
SD rat splenocytes纯化方式
Protein G浓度
0.5 mg/ml标记
FITC性状
Liquid缓冲体系
PBS, 1% BSA, 0.3% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
FCM
稀释度
应用 稀释度 推荐种属 FCM 1μg per million cells in 100μl volume Rt
CD47 is a ubiquitously expressed transmembrane glycoprotein that serves as a critical “don’t-eat-me” signal by engaging signal-regulatory protein α (SIRPα) on macrophages and dendritic cells, thereby inhibiting phagocytosis and bridging innate and adaptive immunity; beyond immune evasion, CD47 modulates integrin activation, cell adhesion, migration, apoptosis, and metabolic signaling, and its marked over-expression on diverse tumor cells—driven by oncogenic pathways such as KRAS-PI3K-STAT3, hypoxia-HIF-1α, or amino-acid-mTOR cascades—correlates with reduced patient survival, making blockade of the CD47–SIRPα axis a promising therapeutic strategy to restore anti-tumor immunity.
流式分析
Flow cytometric analysis of rat CD47 expression on SD rat splenocytes. SD rat splenocytes were stained with APC Mouse Anti-Rat CD3 Antibody and either FITC Mouse IgG1, k Isotype Control (left panel) or SDT FITC Mouse Anti-Rat CD47 Antibody (right panel) at 2 μl/test. Total viable cells, as determined by Fixable Viability Dye 452 (S0D0021), were used for analysis. Flow cytometry and data analysis were performed using Agilent NovoCyte Quanteon and FlowJo™ software.
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